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RNA Modifications

181,89 €*

Sofort verfügbar, Lieferzeit: 1-3 Tage

Produktnummer: 180a214dd3b0594a8b8b31f4fc5a984bdd
Themengebiete: Epitranscriptome Messenger RNA Next-generation sequencing Post-transcriptional RNA modifications Post-transcriptional gene regulation qPCR
Veröffentlichungsdatum: 04.06.2021
EAN: 9781071613733
Sprache: Englisch
Seitenzahl: 427
Produktart: Gebunden
Herausgeber: McMahon, Mary
Verlag: Springer US
Untertitel: Methods and Protocols
Produktinformationen "RNA Modifications"
This detailed book describes some of the most recent advances and up-to-date methodologies to detect, quantify, analyze, and elucidate the biological function of different types of RNA modifications. Importantly, the methodologies and tools described herein can be applied to a wide variety of organisms and can be used to address biological and clinical questions. Beginning with a section on bioinformatics tools, the collection continues with sections on detecting RNA modifications using Nanopore direct RNA sequencing, next-generation sequencing approaches, qPCR- and molecular biology-based methods, mass spectrometry- and NMR-based methods, as well as approaches to assess kinetics, determinants, and functions of RNA modifications. Written for the highly successful Methods in Molecular Biology series style, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Modifications: Methods and Protocols serves as an ideal guide for those working directly in the fields of epitranscriptomics and post-transcriptional gene regulation, as well as for scientists and clinicians interested in bioinformatic tools to study RNA modifications and techniques to dissect their roles in physiology and disease.Chapter 20 is available open access under a CC BY 4.0 license.

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